Monitor immune diversity recovery after treatment Identification of antigen-specific TCR/BCRĮvaluation of the efficacy of immune checkpoint blockers Key Advantages of Rep-seq Service in Creative Biolabs Include but Are Not Limited to:ĭiverse species: human, mouse, rhesus monkey, alpaca, canine, rabbit, swine, feline, zebrafish, etc.Ī Wide Variety of Applications of Rep-seq Service Include but Are Not Limited to: In this way, our customers can finally obtain a comprehensive BCR repertoire involving both the hypermutation and isotype data from this sequencing service. Based on our advanced SuPrecision™ platform, both the heavy chain and the light chain CDR3 regions can be 100% covered and accurately sequenced, the constant regions from the two species are also captured and sequenced with enough length to identify the class information.
RNA from whole blood or B-cells is the required template for the amplification of CDR. Through this excellent platform, our clients can obtain the sequence data of the whole covered CDR3 regions of the interested TCR chain, which has involved most of the hypermutation information. Our SuPrecision™ platform permits RNA as a template to amplify and sequence the complementary determining region (CDR) of whether α, β, γ or δ chain of TCR. Paired-end sequencing with additional PCR primers in the middle of the fragment permits full-length TCR repertoire sequencing with short read NGS technology to resolve the V/J pairing. The V(D)J, and most importantly, the variable complementarity determining region CDR3 sequences, and their respective abundance can be resolved by high-throughput sequencing. Basically, multiplex PCR can amplify the recombined V(D)J regions from either mRNA or DNA in the B- or T-cells. A plethora of immune repertoire methods have been published and commercial solutions are also available. The Background of Immune Repertoire Sequencing (Rep-seq)ĭNA sequencing approaches have enabled the characterization of immune repertoires. Upon recognition of foreign antigens and with the presence of co-stimulatory molecules, B-cells and T-cells express cell surface activation markers, attack foreign antigens, secrete cytokines, stimulate each other, and proliferate. T-cells only recognize foreign proteins presented on MHC, while B-cells can also target foreign DNA, lipids, or carbohydrates. During B and T-cell development, self-antigens are presented to B and T-cells to select out self-reacting types, and to ensure only B and T-cells that recognize and attack foreign antigens are in the circulation. V(D)J recombination in the primary lymphoid organs creates the incredibly diverse and unique repertoire of the hypervariable regions of BCR and TCR, and somatic hypermutations contribute to additional BCR diversity. 2016).Ĭellular immune responses from T-cells and humoral immune responses from B-cells are stimulated by exposures to antigens, including pathogens, allergens, and neoantigens. Depending on our cutting-edged SuPrecision™ platform which mainly based on next-generation sequencing (NGS) technique, our scientists have accumulated extensive experience in identifying the whole four types of T-cell receptor (TCR) chains (α, β, γ, and δ) and the vast majority of B-cell receptor (BCR) V(D)J sequences contained in a single sample.įig.1 Structure, function, and diversification of TCR/BCR receptors (Lossius et al.
Immune Repertoire Sequencing (Rep-Seq) Service for CancerĬreative Biolabs offers the unparalleled mass sequencing service to analyze the immune repertoires for our customers all over the world.
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